Detection and Diagnosis of Cervical Cancer

Cervical cancer originates in the cells lining the cervix, the lower part of the uterus (womb). It can be detected in its early stages, and in some cases, it can even be completely prevented through routine screening tests.

These tests include the HPV (human papillomavirus) test and the Pap test, which can be done individually or together (co-testing).

Pap Test

The Pap test is a procedure where cells from the cervix are collected, fixed onto a slide, and stained to be carefully observed under a microscope to determine if there is cancer or precancer.

HPV Test

In this case, the presence of HPV DNA in cervical cell samples is analyzed using the qPCR test (quantitative polymerase chain reaction). 

Prior to qPCR, DNA extraction from the cell sample must be performed. This process varies depending on the extraction kit used, but generally involves adding a series of reagents and performing sequential centrifugations, first at low intensity (<2,500 RPM) and then at higher intensity (>10,000 RPM), culminating in the isolation of the desired DNA.

For this centrifugation stage, our Cyto 22, specifically designed for applications in oncology, cytology, hematology, virology, and microbiology, is ideal for cervical cancer detection procedures. Its simple operation significantly reduces operation time, an essential feature for handling a high volume of samples.

The isolated DNA after the centrifugation step will be processed using qPCR, and the presence and viral load in the sample will be determined, thus achieving a diagnosis.

Early diagnosis of cervical cancer can significantly improve the chances of effective treatment and prevent the progression of the disease.

To detect HPV with the qPCR test, a centrifugation process is required. This centrifugation step, involving stages at different speeds, ensures the purity and quality of the extracted DNA, allowing for precise and reliable detection via qPCR.