In the face of infectious outbreaks, acting quickly makes all the difference. That’s why science is turning to more precise and faster methods, such as DNA-based microbial typing, which has revolutionized epidemiological studies.

In this advancement, microcentrifuges play a crucial role, as they allow for the extraction and purification of bacterial DNA, a key step in applying techniques such as whole genome sequencing (WGS), which is increasingly used in this field.

Why is Microbial Typing Important in Epidemiology?

Microbial typing helps identify the origin, transmission, and evolution of infectious diseases. In hospitals, it is essential for tracking healthcare-associated infections and preventing their spread. It also helps detect virulent or resistant strains and assess the effectiveness of control measures.

While conventional methods like serotyping or biochemical analysis have been widely used, their low discrimination capacity and limited reproducibility have driven the shift towards more accurate and reliable molecular technologies.

The Role of Microcentrifuges in Bacterial DNA Extraction

DNA extraction is a critical step in any genetic analysis. It requires meticulous preparation, including cell lysis and the separation of DNA from other cellular components.

Microcentrifuges make this process efficient by reaching high speeds (up to 15,000 rpm), which are essential for precise separation. In protocols such as whole genome sequencing (WGS), centrifugation at 8,000 x g is used to obtain a clean supernatant suitable for further analysis.

Microcentrifuges like the Microcen 24, Biocen 22, and Biocen 22 R are all suitable for working with small volumes and optimizing processes such as DNA extraction and purification.

The Biocen 22 R, in particular, stands out for its cooling system, precise temperature control, and adaptability to various protocols, making it an especially valuable tool in molecular microbiology labs.

Whole Genome Sequencing: A High-Precision Tool

Whole genome sequencing (WGS) has become one of the most precise techniques for microbial typing. By analyzing the entire DNA of a pathogen, it provides an unparalleled resolution of its genetic profile, even at the sub-species level.

This technique allows for highly accurate strain comparisons, facilitating the establishment of relationships between outbreaks, tracking the genetic evolution of bacteria, and even identifying sources of infection in humans, animals, or food. WGS has thus become a cornerstone of modern epidemiological surveillance.

The combination of microcentrifuges and sequencing techniques like WGS is transforming modern epidemiology. The precision, speed, and reliability offered by these methods allow for informed decision-making in record time, which is essential for public health protection. Equipment such as the Microcen 24, Biocen 22, and Biocen 22 R are fundamental tools in this process, as they enable efficient and precise DNA extraction and purification. In a world where outbreaks are becoming increasingly complex, having the right tools in the laboratory is not optional, but a necessity.